Services

We provide two photon microscopy to run the following type of in vivo imaging experiments. Sample preparation supplies (Prep Supplies hereinafter) listed under each type of experiment are included in all tier (I, II and III) service price. Other resources such as reagents and reporter mice are available to tier II and III service customers for purchase or can be provided by customers. For details, see Other Resources. If you do not see the type of experiment you would like to conduct in the list below, please contact us and we will assess the possibility case by case.

This is for terminal procedure only.

  • Thoracic: Heart, Lung, Thymus

    •   Anesthesia
      • Ketamine/ xylazine
    •   Surgery equipment
      • Stereo dissection scope
      • Ventilator
      • Tools: clamps, scalpels, scissors, forceps
      • Materials: syringes, tubing (intubation and injection), gauze, gloves, tape, PBS/saline, super glue
    •   Thoracic  imaging chamber:  cover glass, O-rings, heaters
  • Peritoneal

    •  Anesthesia
      • Ketamine/ xylazine
      • Isoflurane and oxygen or carbagen gas, nose cone and tubing
    •  Surgery equipment
      • Stereo dissection scope
      • Tools: clamps, scalpels, scissors, forceps
      • Materials: syringes tubing, gauze, gloves, tape, PBS/saline, super glue
    •  Peritoneal imaging chamber:  cover glass, O-rings, heaters
  • CNS (Central Nervous System – brain and spinal cords)

    •   Anesthesia
      • Ketamine/ xylazine
      • Isoflurane and oxygen or carbagen gas, nose cone and tubing
    •   Surgery equipment
      • Stereo dissection microscope scope
      • Tools: clamps, scalpels, scissors, forceps
      • Materials: syringes, tubing, gauze, gloves, tape, PBS/saline, super glue
    •   Stereo-tactic imaging chamber:  Stereo-taxis device, cover glass, O-rings, heaters
  • Peripheral (lymph nodes, exposed subcutaneous tumors etc.)

    •   Anesthesia
      • Ketamine/ xylazine
      • Isoflurane and oxygen or carbagen gas, nose cone and tubing
    •   Surgery equipment
      • Stereo dissection scope
      • Tools: clamps, scalpels, scissors, forceps
      • Materials: syringes tubing, gauze, gloves, tape, PBS/saline, super glue
    •  Peripheral imaging chamber:  cover glass, O-rings, heaters
  • Foot pad

    •   Anesthesia
      • Ketamine/ xylazine
      • Isoflurane and oxygen or carbagen gas, nose cone and tubing
    •   Surgery equipment
      • Tools: clamps, scalpels, scissors, forceps
      • Materials: syringes tubing, gauze, gloves, tape, PBS/saline, super glue
    •   Foot-pad imaging chamber:  cover glass, O-rings, heaters
  • Ear

    •   Anesthesia
      • Ketamine/ xylazine
      • Isoflurane and oxygen or carbagen gas, nose cone and tubing
    • Surgery equipment
      • Tools: clamps, scalpels, scissors, forceps
      • Materials: syringes tubing, gauze, gloves, tape, PBS/saline, super glue
    •  Ear imaging chamber:  cover glass, O-rings, heater.
  • Other (eye, tongue, etc.)

    •  Anesthesia
      • Ketamine/ xylazine
      • Isoflurane and oxygen or carbagen gas, nose cone and tubing
    •  Surgery equipment
      • Tools: clamps, scalpels, scissors, forceps
      • Materials: syringes tubing, gauze, gloves, tape, PBS/saline, super glue
    • Custom imaging chamber:  cover glass, O-rings, heaters.

This applies to human tissue biopsy or dissected tissues from a euthanized animal.

  • Tissues: lymph nodes, spleen, liver, kidney, lungs etc.

    •     CO2 euthanasia plus cervical dislocation
    •     Surgery equipment
      • Tools: clamps, scalpels, scissors, forceps
      • Materials: syringes tubing, gauze, gloves, tape, PBS/saline, super glue
    •     Ex plant imaging chamber:  cover glass, O-rings, heaters, perfusion media, CO2 independent media, carbagen gas, and tubing.

We have two Dell workstations equipped with the following software and devices.

  • 3-D image rendering, cell tracking, analysis and visualization of multi-dimensional time-lapse data: Imaris, Volocity, T-cell analyzer
  • Statistical analysis and generating publication ready figures: Prism
  • Editing and annotating time-lapse movies: Adobe Premier
  • Microsoft Office
  • Data Backup and Storage Devices